agarose_gel
Agarose Gel Preparation
- Weigh 4g Agarose gel powder (2% gel) into a flask that will be used to make the gel solution
- Add 200 mL of TAE+EtBr solution into the flask
- Microwave the gel mix until all agarose powder has dissolved (approx. 3 min)
- Prepare the gel tray by taping the top/bottom ends
- Cool the solution a little by gentle swirls
- Pour the gel into the tray and set the comb
- Leave gel to set at RT
Tips:
- When heating the gel mix solution in the microwave, give a swirl often to check how much longer you need to heat the solution to avoid over heating
- Avoid having any bubbles in the gel mix solution – otherwise you will get bubbles in the gel!
- There are 22 loading wells (you can load 20 samples/gel, 2 wells for DNA ladder)
- Always load a DNA ladder at each end of the gel
Solutions:
| Tris-acetate EDTA (TAE) with Ethidium bromide (EtBr) (5 L) | |
|---|---|
| 50x TAE stock | 100 mL |
| dH2O (green tap) | 4900 mL |
| EtBr | 16 drops (in the door of Fridge #6A) |
| 50x TAE stock (1 L) | |
|---|---|
| Tris acetate | 242 g |
| Glacial acetic acid | 57.1 mL |
| EDTA (0.5 M; pH 8.0) | 100 mL |
| dH2O (yellow bottle) | make up to 1 L |
agarose_gel.txt · Last modified: 2021/01/19 11:10 by 127.0.0.1