| To prepare 200 ml | Final concentration | |
|---|---|---|
| HEPES 1M (pH 7.0) | 10ml | 50 mM |
| SDS 20% | 1 ml | 0.1% |
| Triton X-100 | 2ml | 1% |
| EDTA 0.5M (pH 8) | 400 µl | 1 mM |
| Deoxycholic acid | 2g | 1% |
| NaCl 5M | 6ml | 150 mM |
Fill to 200 ml with <chem>H2O</chem>, sterile filter, store at RT. Use 1ml per fastprep tube.