User Tools

Site Tools


ripa_buffer

RIPA Buffer

To prepare 200 mlFinal concentration
HEPES 1M (pH 7.0)10ml50 mM
SDS 20%1 ml0.1%
Triton X-1002ml1%
EDTA 0.5M (pH 8)400 µl1 mM
Deoxycholic acid2g1%
NaCl 5M6ml150 mM

Fill to 200 ml with <chem>H2O</chem>, sterile filter, store at RT. Use 1ml per fastprep tube.

ripa_buffer.txt · Last modified: 2020/02/27 18:22 (external edit)