Primo Printing 96 well plate - PEG SVA

Tuesday: 19.06.18.

StockDiluentWorking concentration
5µl 10mM/mL PLL95µl water500µg/ml
5 mg PEG-SVA100µl HEPES (pH 8-8.5)
Printing Protein94ml
  • Add 50µl of Poly-L-Lysine per well
  • Add 300 µl water to peripheral wells
  • Incubate for 30 minutes
  • Rinse 5x with HEPES (100µl per rinse)
  • During rinse step 4/5, dissolve 5 mg PEG-SVA in 50µl HEPES per well), mix with pipette and allow to stand for 1-2 minutes.
  • Add 50µl of PEG-SVA solution to each well
  • Incubate for 1 hour (RT)
  • Rinse 5x with PBS
  • Add 45µl PLPP per well and print

Try to avoid touching the pipette tip off the surface of the glass

Be careful to never allow the surface to dry out


  • Turn on Tweety, Primo, the Zyla camera and finally the PC.
  • Open Micromanager, then load the primo cfg, and go to Plugins>Leanardo to launch Leanardo.
  • Set the PFS with a fluorescent marker-tagged dish/coverslip with the same thickness as the sample dish.
  • Open the 96 well template, place your pattern and run.
  • After the run is complete, rinse 5x with PBS. Add the protein of interest and incubate at RT for 5'.
  • Rinse 5x with PBS.